Optimization of a Validated UV-Spectrophotometric Methodology for Assessment of Apigenin in Bulk Powder

Abstract: Introduction: Apigenin (4,5,7 Trihydroxyflavone) is a natural compound of flavonoids class, which found in various plant constituents. Since primitive years, has been used as traditional medicine, because it possesses biological functions. Common ayurvedic formulations comprising apigenin are market. Hence quality control preparation covering desirable. Objectives: In present study, pointed to enhance and validate UV Spectrophotometric method evaluation bulk powder. Methods: Using methanol solvent, was developed. showed supreme absorbance wavelength at 267 nm. New technique enhanced validated terms parameters such Specificity, Discrimination, Linearity, Correctness, Ruggedness, Solution stability per each ICH guidelines. Results: The detector response for linear the sure concentration range 2μg/mL- 10μg/mL with correlation coefficient 0.9995. Newly developed be specific, Selective linear, Precise, Rugged, reproducible estimation %RSD values less than 2%. Conclusion: this study Development Validation UV-Spectrophotometric can employed assessment Key words: Apigenin, Absorbance, guidelines, Spectrophotometric, Ayurvedic Formulations.